Abstract:OBJECTIVE To investigate the clinical value of serum triiodothyroxine (T3), thyroxine (T4) and thyrotropin (TSH) in patients with thyroid disease. METHODS Eighty patients with thyroid disease admitted from January 2015 to January 2017 were divided into hyperthyroid group (n = 50) and hypothyroidism group (n = 30) according to different types of disease. Fifty patients with normal physical examination were treated as control group. Serum T3, T4 and TSH levels were measured by enzyme immunofluorescence in all patients, and the clinical value of thyroid disease was analyzed. RESULTS Compared with the control group, the levels of T3 and T4 in the patients with hyperthyroidism were significantly increased and the TSH levels were significantly decreased (P＜0.05). Compared with the control group, the T3 level of the patients in the hypothyroid group was significantly lower than that in the control group (P ＜0.05). Compared with the hypothyroidism group, the levels of T4 and TSH were significantly higher than those of the hypothyroid group (P＜0.05), and the difference was statistically significant (P＜0.05) The Conclusion: The thyroid function of patients with thyroid disease can be reflected by the levels of serum T3, T4 and TSH. The above indicators can evaluate thyroid function, which is ideal And accurate method, through the detection of the above indicators can diagnose thyroid disease diagnosis plays a very important role, has a high clinical value.