Abstract:OBJECTIVE To investigate the infection status of human papilloma virus (HPV) in Shanghai Anting Town, and to provide reference for cervical cancer prevention and vaccine development. METHODS The cervical exfoliated cells were collected in population sampled by stratified cluster sampling, and the HPV genotype was detected by gene sequencing. RESULTS 4 781 valid samples were collected, and 484 cases were HPV infected, the infection rate of HPV were 10.12%，among them 465 cases were high risk HPV（HR-HPV） infection，the infection rate of HR-HPV were 9.73%，19 cases were low risk HPV（LR-HPV） infection，the infection rate of LR-HPV were 0.39%.There were 411 cases with single HPV type infection, 73cases with multiple HPV subtypes infection. HPV infection rate in different age groups were different, and 61~65 years women had the highest HPV infection rate. The difference among different age groups was statistically significant (χ2=39.91，P＜0.05). The top four HPV genotypes were HPV52, 58, 16 and 68, and the infection rates respectively were 2.64%, 1.55%, 1.34% and 1.11%. CONCLUSION The HPV infection rate of household registered women in Anting, Shanghai is at a low level. But in the future, it is still necessary to strengthen the health education of HPV and cervical cancer for middle-aged and old women, and continue to promote the development of HPV vaccine.