Abstract:OBJECTIVE To understand college students’ knowledge, attitude and practice on smoking, and to explore the associated factors of cigarette use, so as to provide the basis for formulating relevant measures and building smoke-free campus. METHODS 947 students were recruited using stratified cluster sampling method and finished self-designed questionnaires. Data was analyzed with statistical software SPSS 20.0, and statistical methods included chi square test and logistic regression analysis. RESULTS Among 947 college students, the smoking rate was 10.0%, current smoking rate was 5.1%. The male current smoking rate was 8.1%, higher than the female （χ2=22.920，P<0.05）. The correct answer rate of five questions on smoking harm were 96.5%, 68.2%, 47.7%, 40.9%, 36.7%, respectively, and the awareness rate on smoking harm related knowledge was 53.4%, and of medical students had higher awareness rate (64.10%) than those in other majors. The correct answer rate of four questions on secondhand smoke harm were 81.6%, 85.5%, 43.9%, 68.8%, respectively, and the awareness rate on second-hand smoke harm related knowledge was 64.9%, the awareness rate of the female(72.80%) was higher than male(58.80%). 92.5% of students were in favor of the health hazards of smoke inhalation, and 86.9% of students thought they were worried about their health when someone smoked around them, and 81.7% of students supported the ban on smoking in public places. Attitude towards smoking was related to the major, gender and grade. Male students (OR=5.370，95%CI：2.065～13.969) and those who thought that someone smoking around them would not influence their health(OR=9.967，95%CI：4.978～19.957) were more likely to smoke. CONCLUSION It is necessary to carry out the propaganda of different smoking harms targeted on students with different characteristics, especially strengthening on the second-hand smoke harm, to improve the students' attitude towards smoking, and to reduce the smoking behavior in public places.