Abstract:OBJECTIVE To analyze the blood pressure distribution and three serological test results of neonatal hemolytic disease in diagnosis. METHODS Hemolysis trials of type O or Rh negative maternal cord blood samples and hyperbilirubinemia of the newborn blood samples from January 2013 to November 2018were detected by micro-column gel test cards. Then the results were statistically analyzed. RESULTS There were 82 cases of Maternal and neonatal blood type incompatibility in all 262 cases. 51cases were detected HDN positive with the rate of 62.12%. The positive rate of direct anti-globulin test, free antibody test and antibody released test were 27.45%, 86.27% and 100%. There were statistical difference of HDN positive rate between ABO 73.13% and Rh 13.33% in 82 cases of blood type incompatibility. There was statistical difference between A positive rate(80.65%) and B positive rate(69.44%) in 67 cases of ABO incompatibility. There were statistical difference among Rh D positive rate(14.28%), Rh E positive rate(20%) and Rh C positive rate(0). CONCLUSION Antibody released test was the most sensitive test in hemolysis three trials to diagnose HDN positive the probability of HDN positive caused by maternal and neonatal ABO blood type incompatibility was significantly higher than Rh. The probability of HDN positive with type A newborns was significantly higher than type B. The probability of HDN positive caused by Rh D blood type incompatibility was significantly higher than Rh E and Rh C.