Abstract:OBJECTIVE To investigate the serum levels of Interleukin-6 (IL-6), Interleukin-8 (IL-8) and Tumor Necrosis Fac⁃ tor-TNF-α (TNF-α) and association with chronic gastritis patients with helicobacter pylori infection. METHODS Clinical data of 222 patients with chronic gastritis who visited the hospital from March 2019 to March 2020 were selected. The infection of Helico⁃ bacter pylori was detected by 13C urea breath test and immunohistochemical staining, and serum IL-6, IL-8 and TNF-α levels were detected by immunohistochemistry. RESULTS Serum levels of TNF-α and IL-6 in H. Pylori positive patients were significantly higher than those in H. Pylori negative patients with statistically significant differences (P=0.029). However, there was no statistical difference in IL-8 level between H. pylori positive and negative patients (P=0.7000). Serum TNF-α, IL-6 and IL-8 levels were posi⁃ tively correlated with the severity of chronic gastritis. CONCLUSION The serum levels of TNF-α and IL-6 were significantly in⁃ creased in patients with chronic gastritis infected with H. Pylori, but the level of IL-8 was not significantly changed. High serum lev⁃ els of TNF-α, IL-6 and IL-8 were closely related to the severity of gastritis.