Abstract:OBJECTIVE To investigate the effect of microbial enteral nutrition on intestinal microflora, cell and humoral immune function and complication in patients with colorectal cancer. METHODS 90 patients underwent radical resection of colorectal cancer were enrolled and randomly divided into2 groups, 45 cases in each group. The group A was given enteral nutrition combined with probiotics, while the group B was given routine parenteral nutrition. The changes of intestinal flora, lymphocyte subsets, immunoglobulin, serum inflammatory factors and postoperative complications were compared between the two groups. RESULTS There were no significant differences in intestinal flora, lymphocyte subsets, immunoglobulin and serum inflammatory factors before treatment (P＞0.05). The ratios of Bifidobacterium, Lactobacillus, Bifidobacterium to Escherichia (B/E) were all decreased in the two groups after operation (P＜0.05), Escherichia coli and Enterococcus increased (P＜0.05), and the difference between the two groups was statistically significant P＜(0.05). The expressions of CD3+ cells, CD4+ cells, CD8+ cells, CD4+/CD8+, IgG, IgM and IgA were all decreased in the two groups after operation (P＜0.05), and lower in group B than in group A (P＜0.05). The levels of CRP, TNF-α and IL-6 were all increased in the two groups after operation (P＜0.05), and group B increased higher than those in group A (P＜0.05). There was no significant difference in postoperative complications between the two groups (19.05%, 23.26%, respectively; P＞0.05). CONCLUSION The application of microbial enteral nutrition in patients after colorectal cancer can help correct intestinal flora imbalance, improve immune function and reduce serum inflammatory factors, and it have no obvious effect on postoperative complications.