Abstract:OBJECTIVE Research on current situation and the influencing factors of breast-self examination (BSE)among female nursing students in Haikou, in order to provide evidence for health education in the future and improve their health condition METHOD 415 female nursing student are selected from College of nursing ,Hainan Medical university by using cluster sampling, And then carrying out research by using questionnair. RESULTS 390 female nursing students included in the program: there are 21( 15.22%)students among first grade, 39 students(27.08%) among sophomores and 68(62.96%) among junior students. The average SBE rate is 32.82%, there are 32（8.21%）students who can .implement standard SBE procedure.(onceamothatleast ) .Theaveragescoreofbreastcancerelatedknowledge was 45.80  ± 6.73 points, theaveragescoreof attitude and confidence for breastbcancer prevention was 69.99±10.36.The implementation rate of BSE goes up with the grade, and the difference among grades has significance meaning statistically (X2=89.851，P＜0.001);the implementation rate of BSE of those students who had breast disease history is higher than those who had not, and the difference has significance meaning statistically (X2=15.126，P＜0.005). Mother's education background of those students has no significance influence statistically on their SBE implement rate. (X2=0.428，P＞0.05). Logistic analysis shows: grade and breast disease history were the influencing factors of BES for nursing female students, but mother's educational background has no influence on the implementation rate of BSE. CONCLUSION Nursing students master a certain level knowledge of breast diseases and SBE, but the SBE implementation rate still need to be improved. Thus, effective approaches to strengthen the propaganda and Health education about breast diseases and BES is required.