Abstract:OBJECTIVE To explore the significance of the changes of peripheral blood lymphocyte subgroups in patients with cervical high-risk HPV infection. METHODS From August 2014 to August 2016, 373 patients with positive HPV genotyping were research group , and 85 patients with negative HPV were control group. The number of lymphocyte subgroups (CD3 +, CD4 +, CD8 +) in peripheral blood of each group was detected by flow cytometry, and CD4+/CD8+ ratio was calculated. In the research group, the detection results of T cell subgroups of patients with different CIN types, cervical cancer staging, tissue differentiation and lymph node metastasis were compared.RESULTS In research group, the peripheral blood CD3 +, CD4 + lymphocyte subgroup amount is lower than the control group, CD8 + lymphocytes subgroup number higher than that of control group, CD4 + / CD8 + ratio is lower than the control group, the comparative difference were statistically significant (P﹤0.05). With the increase of CIN grade, cervical cancer staging, CD3 +, CD4 + lymphocytes in peripheral blood gradually decline, the number of the group of CD8 + lymphocytes subgroup amount increase gradually, the CD4 + / CD8 + ratio gradually reduce, compare the difference between groups has statistically significant (P﹤0.05). The degree of differentiation, lymph node metastasis positive patients were lower, the number of peripheral blood CD4 + lymphocyte subgroup was lower, the number of CD8 + lymphocytes subgroup was higher, CD4 + / CD8 + ratio was lower, the difference between groups have statistically significant (P﹤0.05).CONCLUSION The detection of lymphocyte subgroup and CD4+/CD8+ ratio can help predict the return of HPV infection.