Abstract:OBJECTIVE The detection of serum 1,25-(OH)2VitD3 and its immune function in children with rotavirus enteritis provide basis for clinical diagnosis and treatment of rotavirus enteritis. METHODS 230 children with rotavirus enteritis from June 2017 to June 2019 were selected as the study objects. 112 children with bacterial enteritis were selected as control group. The levels of procal⁃ citonin (PCT), C-reactive protein (CRP), IL-2 (IL-2), IL-4 (IL-4), 25 hydroxyvitamin D3 (1,25-(OH)2VitD3), immunoglobulin A (IGA), immunoglobulin G (IgG) and immunoglobulin M (IgM) IgM were measured and compared between the two groups, and the rela⁃ tionship between rotavirus enteritis and the above indexes was analyzed. RESULTS The levels of PCT, CRP , AGEs, IL-2 in the study group were significantly higher than the control group, and the levels of 1,25-(OH)2VitD3, IgA, IgG and IgM in the study group were significantly lower than the control group（P<0.05). The positive rates of CRP and PCT in the study group were significantly higher than the control group（P<0.05). Spearman correlation analysis showed that the levels of PCT, CRP, IL-2, ages were positively correlat⁃ ed with rotavirus infection (r=0.520, 0.236, 0.407, 0371, P<0.05). 1,25-(OH)2 VitD3, IgA, IgG and IgM levels were negatively corre⁃ lated with rotavirus infection (r=-0.634, -0.517, -0.564, -0.602, P<0.05). CONCLUSION The decrease of 25-(OH)2VitD3 level could affect the normal immune function of children and increase the incidence of rotavirus infection enteritis. Effective supplementation of 25-(OH)2VitD3 may provide a new way for the prevention and treatment of rotavirus infective enteritis.