Abstract:OBJECTIVE To understand the status of health literacy level of primary school students in Guangzhou before and af⁃ ter comprehensive intervention on health literacy， evaluating the effect of comprehensive intervention on health literacy. METHODS A Stratified random cluster sampling method was used to select a fifth grade class from 10 primary schools in Guangzhou for inter⁃ vention. The number of students intervened were 399. Before and after comprehensive health literacy intervention，the students were evaluated by questionnaire survey. RESULTS The total Health literacy was 74.69% after intervention，which was higher than that be⁃ fore intervention （57.39%），and the difference was statistically significant （P＜0.01）. After the intervention，the health literacy level of basic knowledge and concept，healthy lifestyle and behavior，and basic skills was 75.69%，71.43%，and 84.71%，respec⁃ tively. Before and after the intervention，the differences in the health literacy level of basic knowledge and concepts and basic skills had statistical significance （χ2=27.002, 6.016, P＜0.05）. In the past 6 months，the most common emotion was good emotion，and 19.30% of the respondents felt stress. More than 83.96% of the respondents would take the initiative to seek help in psychological problems，a small number of people took a negative way to alleviate psychological problems. There were a few people who did not confide in anyone （17.29% and 13.78%）. CONCLUSION The level of health literacy of primary school students after intervention was higher than that before intervention. Behavior changed more slowly than knowledge and skills.The improvement effect of health behavior and lifestyle and mental health were not significant. Some primary school students had insufficient sleep， lack of time for outdoor activities，bad mood，psychological problems that they did not find someone to talk to and other problems.