Abstract:Abstract: OBJECTIVE To monitor the resistance trend of Neisseria gonorrhoeae to 7 antibiotics and the genotyping characteristics of N. gonorrhoeae multi-antigen in Yinchuan in 2023, so as to provide laboratory basis for effective selection of antibiotics in clinic. METHODS A total of 21 strains of N. gonorrhoeae were collected, and their drug sensitivity to 7 common antibiotics was detected by agar dilution method. Based on genome-wide sequencing technology, to predict drug resistance phenotype and analyze NG-MAST type, and the coincidence rate between in vitro Antimicrobial Susceptibility Test (AST) and drug resistance phenotype prediction was compared to explore the genotype of N. gonorrhoeae in Yinchuan. RESULTS No isolate was resistant to CFM, CRO, AZM, SPT , PEN and TET were 80.96% and 76.19% . AST and drug resistance phenotype prediction of PEN, TET, CFM, SPT and AZM were all consistent with 100%, and CRO was 80.95%. After sequencing the whole gene, there were 11 and 7 alleles of porB and tbpB, respectively, among which porB 64 and tbpB 2 565 accounted for the most. There are 12 types of NG-MAST. CONCLUSION CFM, CRO, AZM and SPT can still be used as the first choice drugs for gonorrhea patients in Yinchuan, but long-term monitoring is needed to strengthen the attention of AZM, CRO and CFM low-sensitive strains in the area. Typological analysis showed that there were many strains with new genotype of NG-MAST in Yinchuan.